Note the cas3-positive neurons in layer IV (above layer V CTIP2 + neurons). Cortical layers are indicated by Roman numerals. ( C) Immunofluorescence on cortical sections with antibodies against cleaved caspase 3 (cas3, green) and CTIP2 (red) to identify layer V neurons (n = 3 mice). Note the similarity in neurodegeneration between mutant genotypes. Higher magnification images of the region indicated by the rectangle are shown for each genotype. ( B) Hematoxylin and eosin-stained sagittal sections of the DG (n ≥ 3 mice/genotype). Sections were counterstained with DAPI, (n = 3 mice/genotype). ( A) Immunofluorescence of hippocampal sections of the dentate gyrus (DG) with antibodies against cleaved caspase 3 (cas3, green arrowheads indicate positive cells). One-way ANOVA was corrected for multiple comparisons using Tukey method ( G, H). Scale bars: 20 μm ( B, C, D) 500 μm and 50 μm (higher magnification) ( E, F). ML, molecular cell layer PL, Purkinje cell layer GCL, granule cell layer. ( G, H) Number of cerebellar granule cells in lobule VI ( G) and lobule IX ( H) of 3-, 4-, and 6-week-old BP1 (B6J. Cerebellar lobes are indicated by Roman numerals. Higher magnification images of lobule IX are shown below each genotype. ( E, F) Hematoxylin and eosin staining of sagittal sections of the cerebellum (n = 3–4 mice/genotype).
Images of individual probes from areas defined by rectangles in C and D are shown above merged images.
( B–D) In situ hybridization demonstrating ubiquitous expression of Gtpbp1 (yellow) and Gtpbp2 (red) in the P28 wild type (B6J) cerebellum ( B), and CA1 region of the hippocampus ( C), dentate gyrus (DG) ( D) (n = 2 mice). The percent of identical amino acids for each domain is shown. ( A) Domain structure of mouse GTPBP2 and GTPBP1.